Documents
55269-U
HybridSPE®-Phospholipid Ultra
cartridge, bed wt. 30 mg, volume 1 mL, pk of 100
Recommended Products
material
PE frit (5-9 μm)
polypropylene hardware
Quality Level
composition
bed wt., 30 mg
packaging
pk of 100
technique(s)
solid phase extraction (SPE): suitable
volume
1 mL
matrix active group
zirconia-based phase
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Related Categories
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This Item | 52813-U | 1.52013 | 1.52325 |
|---|---|---|---|
| composition bed wt., 30 mg | composition - | composition - | composition - |
| packaging pk of 100 | packaging - | packaging pkg of 3 ea | packaging pack of 3 ea |
| volume 1 mL | volume - | volume - | volume - |
| matrix active group zirconia-based phase | matrix active group zirconia-based phase | matrix active group C8 bonding phase | matrix active group C18 bonding phase, octadecylsilane (ODS) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
General description
The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.
Features and Benefits
- Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
- Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
- 2-3 step generic procedure
- Minimal to no method development
- Available in 96-well and 1 mL cartridge dimensions
- Ultra cartridge allows the use of "In-cartridge" protein precipitation method
- Filtrate is ultra clean, free of fines, and suitable for direct injection in UHPLC-type applications
Legal Information
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Customers Also Viewed
Articles
This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.
We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.
Protocols
A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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