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575656-U

Supelco

HybridSPE®-Phospholipid

96-well Plate, bed wt. 50 mg, volume 2 mL, pk of 1

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NACRES:
NB.21

Quality Level

product line

HybridSPE®

form

solid

composition

bed wt., 50 mg

packaging

pk of 1

technique(s)

solid phase extraction (SPE): suitable

volume

2 mL

matrix active group

zirconia-based phase

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This Item
52798-U55261-U55267-U
vibrant-m

575656-U

HybridSPE®-Phospholipid

vibrant-m

52798-U

HybridSPE®-Phospholipid

vibrant-m

55261-U

HybridSPE®-Phospholipid

vibrant-m

55267-U

HybridSPE®-Phospholipid

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

packaging

pk of 1

packaging

pk of 20

packaging

pk of 100

packaging

pk of 30

composition

bed wt., 50 mg

composition

bed wt., 15 mg

composition

bed wt., 30 mg

composition

bed wt., 500 mg

product line

HybridSPE®

product line

-

product line

HybridSPE®

product line

HybridSPE®

form

solid

form

solid

form

-

form

-

General description

HybridSPE-Phospholipid technology is a simple and generic sample prep platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis. Biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is loaded on the HybridSPE-Phospholipid 96-well plate or cartridge which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids. The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE-Phospholipid stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS-MS analysis.

The "In-well" and "In-cartridge" precipitation methods are available for the HybridSPE-Phospholipid 96-well version and HybridSPE-Phospholipid Ultra cartridge in which biological plasma/serum is first added to either the well or cartridge, followed by acidified acetonitrile (precipitation agent). After a brief mixing/vortexing step, vacuum is applied. Because the 96-well and Ultra cartridge versions contain a series of low porosity hydrophobic filters/frits, the packed-bed filter/frit assembly acts as a depth filter facilitating the concurrent removal of both phospholipids and precipitated proteins during the extraction process. Standard HybridSPE-Phospholipid cartridges require an "off-line" precipitation method.

Features and Benefits

  • Merges the simplicity of protein precipitation and the selectivity of SPE via the targeted removal of phospholipids
  • Reduce ion-suppression through the complete removal of phospholipids and precipitated proteins
  • 2-3 step generic procedure
  • Minimal to no method development
  • Available in 96-well and 1 mL cartridge dimensions

Legal Information

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Customers Also Viewed

Identification and elimination of ion suppression in the quantitative analysis of sirolimus in human blood by LC/ESI-MS/MS
Mano, N., et al.
Journal of Chromatography. B, Biomedical Applications, 879 (13-14), 968-974 (2011)
Improved sensitivity of sedimentary phospholipid analysis resulting from a novel extract cleanup strategy
Zhu, Z., et al.
Organic Geochemistry, 65, 46-52 (2013)
Investigation of endogenous blood plasma phospholipids, cholesterol and glycerides that contribute to matrix effects in bioanalysis by liquid chromatography/mass spectrometry
Ismaiel, O., et al.
Journal of Chromatography. B, Biomedical Applications, 878 (31), 3303-3316 (2010)
Determination of carboplatin in human plasma using HybridSPE-precipitation along with liquid chromatography-tandam mass spectrometry
Hongliang, J,, et al.
Journal of Chromatography. B, Biomedical Applications, 879 (22), 2162-2170 (2011)
Pankaj K Kanaujia et al.
Journal of chromatography. A, 1218(38), 6612-6620 (2011-08-25)
Selective extraction and enrichment of nerve agent degradation products has been achieved using zirconia based commercial solid-phase extraction cartridges. Target analytes were O-alkyl alkylphosphonic acids and alkylphosphonic acids, the environmental markers of nerve agents such as sarin, soman and VX.

Articles

This Sigma-Aldrich article continues to detail new methodology for the analysis of Vitamin D metabolites using HybridSPE-Phospholipid technology.

This study demonstrates the impact of various mobile phase modifiers on the separation; formate modifiers outperform acetate in terms of MS signals (or sensitivity) and chromatographic resolution.

We are presenting an article focusing on ion-suppression and phospholipid contamination and some of their major causes and difficulties.

This Sigma-Aldrich article discusses how the HybridSPE-Phospholipid Technology works and how the phospholipids are removed.

Protocols

This study demonstrates the analysis of Warfarin in plasma samples utilizing chiral and achiral (reversed-phase) LC-MS and effective sample prep to remove endogenous phospholipids

A simple method to enrich phospholipids from plasma samples, involving a HybridSPE-PPT 96-well plate that both retains phospholipids and removes precipitated proteins.

The conversion of clinical methods to LC/MS/MS offers advantages; however, is accompanied by a few limitations, notably interference effects from the endogenous sample matrix.

Chromatograms

application for HPLCapplication for SPE, application for LC-MSapplication for HPLCapplication for HPLCShow More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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