G005854

LC/MS Analysis of Antiarrhythmic Drugs and Metabolites in Plasma on Ascentis^® Express HILIC following Sample Prep using HybridSPE^®-Phospholipid

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: rabbit plasma, unfiltered K2-EDTA, spiked with each compound at 100 ng/mL (3:1, plasma:1% formic acid in acetonitrile); SPE tube/cartridge: HybridSPE-Phospholipid, 96-well plate (575656-U); sample addition: to each well add 100 μL plasma, followed by a 300 μL of 1% formic acid in acetonitrile; elution: collect, filtrate and analyze directly.; column: Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U); mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in acetonitrile, 5:95 (A:B), adjusted to pH 7.0 with formic acid; flow rate: 0.4 mL/min; pressure: 1305 psi (90 bar); column temp.: 35 °C; detector: ESI+, 100-1000 m/z; injection: 0.5 μL
• suitability: application for HPLC
• application(s): clinical

Description

Analysis Note

This application shows the extraction and analysis of antiarrhythmic cardiac drugs in plasma samples using LC/MS/MS. HILIC chromatography on Ascentis Express along with a selective sample preparation using HybridSPE-Phospholipid provided sensitivity and resolution. The highest grade LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Lidocaine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
Amiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
N-Desethylamiodarone hydrochloride solution, 1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant^®
(±)-Flecainide solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®