MilliporeSigma
All Photos(3)

575659-U

Supelco

HybridSPE®-PLus 96-Well Plate

volume 2 mL, pk of 1 ea

Synonym(s):
HybridSPE®-PLus
NACRES:
NB.21

description

96-well Plate

Quality Level

product line

HybridSPE®

composition

bed wt., 50 mg

packaging

pk of 1 ea

technique(s)

solid phase extraction (SPE): suitable

volume

2 mL

matrix active group

zirconia-based phase

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This Item
575673-U57495-U66875-U
packaging

pk of 1 ea

packaging

pk of 20 ea

packaging

pk of 1 ea

packaging

pk of 1

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

technique(s)

solid phase extraction (SPE): suitable

matrix active group

zirconia-based phase

matrix active group

zirconia-based phase

matrix active group

-

matrix active group

C18

description

96-well Plate

description

96-well Plate

description

detection: MS friendly

description

-

product line

HybridSPE®

product line

HybridSPE®

product line

-

product line

-

General description

HybridSPE-PLus is the next generation of HybridSPE-Phospholipid technology. The first of its kind, HybridSPE-Phospholipid technology was first introduced in 2008. It fuses the simple, standardized methodology of traditional protein precipitation with the specificity of solid phase extraction (SPE) for the simultaneous removal of proteins and phospholipids from biological samples prior to LC-MS analysis. Unlike other phospholipid removal products that use a hydrophobic retention mechanism to remove phsopholipids from biological samples, HybridSPE-Phospholipid technology uses a unique Lewis acid/base retention mechanism that allows it to separate phospholipids from even very hydrophobic analytes, such as vitamins, which are often retained along with phospholipids on competitive products.

Our HybridSPE-PLus products offer the same time-proven chemistry of our traditional HybridSPE-Phospholipid products coupled with manufacturing enhancements that stem from years of production experience. These manufacturing enhancements have allowed us to increase flow consistency and reduce sample hold up volumes, further improving analyte recoveries and assay reproducibility. We invite customers to take advantage of the newest technology; HybridSPE-PLus.

Features and Benefits

  • Simple 2-3 step standard methodology analogous to traditional protein precipitation provides simultaneous removal of both proteins and phospholipids
  • Unique Lewis acid/base retention mechanism effectively removes phospholipids from both hydrophilic and hydrophobic analytes of interest (unlike competitive chemistries) eliminating phospholipid-induced ion suppression
  • Highly reproducible, consistent product performance
  • Provides cleaner samples for longer column life and less instrument downtime
  • Available in 96-well and 1 mL cartridge dimensions

Legal Information

HybridSPE is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Small Molecule HPLC

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Solid Phase Extraction (SPE)

Solid phase extraction (SPE) is a technique used for rapid, selective sample preparation and purification prior to chromatographic analysis (e.g. HPLC, GC, TLC). SPE is used to exchange sample matrices, concentrate analytes, and remove interferences and contaminants to improve data and protect the analytical column.

Chromatograms

application for HPLC, application for SPE

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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