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813351

TSKgel® Octadecyl-4PW HPLC Column

phase C18 (octadecyl), L × I.D. 15 cm × 4.6 mm, 7 μm particle size

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Synonym(s):
TSKgel® Reversed Phase HPLC Column (Polymer-based)
eCl@ss:
32110501

material

stainless steel

product line

TSKgel®

manufacturer/tradename

Tosoh 13351

extent of labeling

1 meq/mL ligand density (estimate)

parameter

no limitation salt concentration
0-100% organic solvent
1.2 mL/min flow rate
120 bar max. pressure
5-50 °C temperature

technique(s)

HPLC: suitable

L × I.D.

15 cm × 4.6 mm

matrix

polymer particle platform

matrix active group

C18 (octadecyl) phase

particle size

7 μm

pore size

500 Å pore diameter

operating pH

2-12

separation technique

reversed phase

General description

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500Ã… pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
  • Octadecyl-2PW particles have 125Å pores
  • Octadecyl-4PW particles have 500Å pores
  • Phenyl-5PW RP particles have 1000Å pores
  • Octadecyl-NPR particles are nonporous

Physical form

Shipped in 60% methanol - 40% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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