G005891
HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 μm particles (50538-U)
mobile phase: [A] acetonitrile; [B] methanol; (95:5, A:B)
flow rate: 1.5 mL/min
pressure: 1726 psi (119 bar)
column temp.: 35 °C
detector: UV, 265 nm
injection: 10 μL
sample: 50 μg/mL in 90:10, acetonitrile:methanol
suitability
application for HPLC
application(s)
clinical
vitamins, nutraceuticals, and natural products
Analysis Note
Legal Information
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Analytical column
Standard
Certificates of Analysis (COA)
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