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  • Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips.

Uclacyanin Proteins Are Required for Lignified Nanodomain Formation within Casparian Strips.

Current biology : CB (2020-08-29)
Guilhem Reyt, Zhenfei Chao, Paulina Flis, Isai Salas-González, Gabriel Castrillo, Dai-Yin Chao, David E Salt
ABSTRACT

Casparian strips (CSs) are cell wall modifications of vascular plants restricting extracellular free diffusion into and out of the vascular system [1]. This barrier plays a critical role in controlling the acquisition of nutrients and water necessary for normal plant development [2-5]. CSs are formed by the precise deposition of a band of lignin approximately 2 μm wide and 150 nm thick spanning the apoplastic space between adjacent endodermal cells [6, 7]. Here, we identified a copper-containing protein, Uclacyanin1 (UCC1), that is sub-compartmentalized within the CS. UCC1 forms a central CS nanodomain in comparison with other CS-located proteins that are found to be mainly accumulated at the periphery of the CS. We found that loss-of-function of two uclacyanins (UCC1 and UCC2) reduces lignification specifically in this central CS nanodomain, revealing a nano-compartmentalized machinery for lignin polymerization. This loss of lignification leads to increased endodermal permeability and, consequently, to a loss of mineral nutrient homeostasis.

MATERIALS
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