Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.

Social isolation-related depression accelerates ethanol intake via microglia-derived neuroinflammation.

Science advances (2021-11-06)

Jin-Seok Lee, Sung-Bae Lee, Dong-Woon Kim, Nara Shin, Seon-Ju Jeong, Chae-Ha Yang, Chang-Gue Son

ABSTRACT

[Figure: see text].

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O111:B4, purified by phenol extraction

Supelco

Bicinchoninic Acid solution

Roche

cOmplete^™, Mini Protease Inhibitor Cocktail, Tablets provided in a glass vial

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination, for 200-1000 μg/ml protein

Sigma-Aldrich

RIPA Buffer

Sigma-Aldrich

DAPI, for nucleic acid staining