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  • Extraction of lipase and protease and characterization of activated sludge from pulp and paper industry.

Extraction of lipase and protease and characterization of activated sludge from pulp and paper industry.

Preparative biochemistry & biotechnology (2013-01-11)
Santosh Kr Karn, Pradeep Kumar, Xiangliang Pan
ABSTRACT

Hydrolytic enzymes released by the microorganisms in activated sludge are responsible for the organic matter degradation there; however, the optimal extraction procedure of this valuable resource has not been well established until now. In this study protease and lipase were extracted from activated sludge using ultrasound disintegration combined with a nonionic detergent (Triton X-100) and cation-exchange resin (CER) in combination for the extraction of protease and lipase. It was observed that the concentration of 0.1% and 1% Triton X-100 has a strong influence for the extraction of lipase and protease respectively. Closer study of the enzyme extraction process is essential for different enzymes from activated sludge process.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100 solution, BioUltra, for molecular biology, ~10% in H2O
Sigma-Aldrich
Triton X-305 solution, 70% in H2O
Sigma-Aldrich
Triton X-102
Octoxinol 10, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
Triton X-45
Sigma-Aldrich
Triton X-100, peroxide- and carbonyl-free
Sigma-Aldrich
Triton X-100, BioXtra
Sigma-Aldrich
Triton X-100, for molecular biology