Skip to Content
MilliporeSigma
  • Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Clinical chemistry (1989-04-01)
G Hoffmann, S Aramaki, E Blum-Hoffmann, W L Nyhan, L Sweetman
ABSTRACT

This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-Hydroxyphenylacetic acid, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Palmitic acid, ≥98%, FCC, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
L-(+)-Lactic acid solution, in H2O, ≥85%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
α-Ketoglutaric acid, 99.0-101.0% (T)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glycolic acid solution, high purity, 70 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glycolic acid solution, technical grade, 70 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Palmitic acid, analytical standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
L-(+)-Lactic acid solution, meets analytical specification of Ph. Eur., BP, 88-92% total acid basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
L-(+)-Lactic acid solution, ~40% in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
3-Hydroxybutyric acid, 95%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Hippuric acid, 98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
L-(+)-Lactic acid solution, 30% in H2O (by weight)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Homovanillic acid, Fluorimetric reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
α-Ketoglutaric acid, BioReagent, suitable for cell culture, suitable for insect cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
cis-Aconitic acid, ≥98%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Palmitic acid, BioXtra, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
α-Ketoglutaric acid, ≥98.5% (NaOH, titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Palmitic acid, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glycolic acid, SAJ special grade, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glycolic acid solution, CP, 70% in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Mettler-Toledo Calibration substance ME 18555, Benzoic acid, analytical standard, (for the calibration of the melting point system), traceable to primary standards (LGC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Palmitic acid, ≥98% palmitic acid basis (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Palmitic acid, European Pharmacopoeia (EP) Reference Standard
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Palmitic acid, natural, 98%, FG
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Palmitic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Palmitic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Glutaric acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Supelco
Glycolic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glutaric acid, 99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00
Sigma-Aldrich
Glycolic acid, BioXtra, ≥98.0% (titration)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
1 g
In Stock
Details...
$70.30
5 g
In Stock
Details...
$256.00
25 g
In Stock
Details...
$971.00