Skip to Content
MilliporeSigma
  • Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine.

Antagonistic effects of indoloquinazoline alkaloids on antimycobacterial activity of evocarpine.

Journal of applied microbiology (2015-01-22)
C Hochfellner, D Evangelopoulos, M Zloh, A Wube, J D Guzman, T D McHugh, O Kunert, S Bhakta, F Bucar
ABSTRACT

The interaction of quinolone and indoloquinazoline alkaloids concerning their antimycobacterial activity was studied. The antimycobacterial and modulating activity of evodiamine (1), rutaecarpine (2) and evocarpine (3) was tested on mycobacteria including three multidrug-resistant (MDR) clinical isolates of Mycobacterium tuberculosis. Antagonistic effects were concluded from fractional inhibitory concentration (FICI) values. Interaction energies of the compounds were calculated using GLUE docking module implemented in GRID. 1 and 2 exhibited weak inhibition of rapidly growing mycobacteria, however, 1 was active against Myco. tuberculosis H37Rv (MIC = 10 mg l(-1) ) while 2 was inactive. Both 1 and 2 showed a marked antagonistic effect on the susceptibility of different mycobacterial strains to 3 giving FICI values between 5 and 9. The interaction energies between compounds 1 and 2 with compound 3 suggested the possibility of complex formation in solution. Indoloquinazoline alkaloids markedly reduce the antimycobacterial effect of the quinolone alkaloid evocarpine. Complex formation may play a role in the attenuation of its antimycobacterial activity. This study gives a striking example of antagonism between compounds present in the same plant extract which should be considered in natural product based screening projects.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol solution, 83.5-89.5% (T)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%
SKU
Pack Size
Availability
Price
Quantity
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Isoniazid, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Supelco
Glycerol, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Oleic acid, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Supelco
Oleic acid, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, BioUltra, for molecular biology, anhydrous, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, tested according to Ph. Eur., anhydrous
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Oleic acid, technical grade, 90%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, for molecular biology, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, BioXtra, ≥99% (GC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Isoniazid, analytical standard, ≥99% (TLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerol, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Glycerin, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Oleic acid, ≥99% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Oleic acid, BioReagent, suitable for cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
β-D-Allose, rare aldohexose sugar
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dimethyl sulfoxide, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
SKU
Pack Size
Availability
Price
Quantity