Skip to Content
MilliporeSigma
  • Wild celery (Smyrnium olusatrum L.) oil and isofuranodiene induce apoptosis in human colon carcinoma cells.

Wild celery (Smyrnium olusatrum L.) oil and isofuranodiene induce apoptosis in human colon carcinoma cells.

Fitoterapia (2014-06-14)
Luana Quassinti, Filippo Maggi, Luciano Barboni, Massimo Ricciutelli, Manuela Cortese, Fabrizio Papa, Chiara Garulli, Cristina Kalogris, Sauro Vittori, Massimo Bramucci
ABSTRACT

Smyrnium olusatrum (Apiaceae), well known as wild celery, is a biennal celery-scented plant used for many centuries as a vegetable, then abandoned after the introduction of celery. In the present work, the essential oil obtained from inflorescences and the amounts of its main constituents isofuranodiene, curzerene and germacrone were analyzed by GC as well as by HPLC because of their degradation (Cope rearrangement) occurring at high temperatures. The oil and the main constituents were assayed for cytotoxic activity on the human colon cancer cell line (HCT116) by MTT assay. Flower oil and isofuranodiene showed noteworthy activity on tumor cells with IC50 of 10.71 and 15.06 μg/ml, respectively. Analysis of the cytotoxic activity showed that wild celery oil and isofuranodiene are able to induce apoptosis in colon cancer cells in a time and concentration-dependent manner suggesting a potential role as models for the development of chemopreventive agents.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetonitrile solution, contains 10.0% acetone, 0.05% formic acid, 40.0% 2-propanol
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (w/v) ammonium formate, 5 % (v/v) water, 0.1 % (v/v) formic acid, suitable for HPLC
Sigma-Aldrich
Triacontane, 98%
Sigma-Aldrich
Docosane, 99%
Sigma-Aldrich
Linalool, ≥97%, FCC, FG
Sigma-Aldrich
Myrcene, stabilized, FCC, FG
Sigma-Aldrich
Tricosane, 99%
Sigma-Aldrich
p-Cymene, 99%
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Sigma-Aldrich
Acetonitrile solution, contains 0.05 % (v/v) trifluoroacetic acid
Sigma-Aldrich
Acetonitrile solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
Supelco
Myrcene, analytical standard
Sigma-Aldrich
p-Cymene, ≥97%, FG
Sigma-Aldrich
Heneicosane, 98%
Sigma-Aldrich
Linalool, 97%
Sigma-Aldrich
Myrcene, technical grade
Supelco
p-Cymene, analytical standard
Supelco
Linalool, analytical standard
Supelco
Triacontane, analytical standard
Supelco
Heneicosane, analytical standard
Supelco
Docosane, analytical standard
Supelco
Tricosane, analytical standard
Supelco
Residual Solvent - Acetonitrile, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Hexane, JIS special grade, ≥96.0%
Sigma-Aldrich
Hexane, HPLC Plus, for HPLC, GC, and residue analysis, ≥95%
Sigma-Aldrich
Acetonitrile, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Acetonitrile, for residue analysis, JIS 5000
Sigma-Aldrich
Hexane, for residue analysis, JIS 5000
Sigma-Aldrich
Hexane, suitable for HPLC
Sigma-Aldrich
Acetonitrile, for chromatography