Skip to Content
MilliporeSigma
  • Protection of cultured human hepatocytes from hydrogen peroxide‑induced apoptosis by relaxin‑3.

Protection of cultured human hepatocytes from hydrogen peroxide‑induced apoptosis by relaxin‑3.

Molecular medicine reports (2014-11-06)
Xiao Ma, Su Han, Wei Zhang, Yu-Jing Fan, Ming-Na Liu, Ai-Yun Liu, Bing-Rong Liu
ABSTRACT

Previous studies have suggested that hepatocyte apoptosis may be a fundamental underlying mechanism of liver injury and diseases, such as liver fibrosis. Relaxin‑3 has been reported to have anti‑fibrotic actions in the heart and to attenuate isoproterenol‑induced myocardial injury; however, the beneficial role of relaxin‑3 on hepatocyte apoptosis remains to be elucidated. The aim of the present study was to explore the role and possible mechanisms of relaxin‑3 through hydrogen peroxide (H2O2)‑induced apoptosis in primary human hepatocytes. Cells were treated with relaxin‑3 and then cell viability, morphological features, the presence of cleaved caspases as well as the levels of endoplasmic reticulum stress (ERS) protein markers and autophagy markers were evaluated. The H2O2 group showed significantly decreased cell viability, increased apoptosis as well as upregulation of caspases (cleaved caspase‑3, ‑8 and ‑9) and ERS protein markers compared with those of the control group. However, cells treated with relaxin‑3 (10 ng/ml) demonstrated improved cell viability, reduced apoptosis and decreased expression of cleaved caspases and ERS markers. However, the expression of autophagy markers remained unchanged following H2O2‑induced apoptosis and relaxin‑3 treatment. In conclusion, relaxin‑3 was shown to protect hepatocytes from H2O2‑induced apoptosis via downregulation of cleaved caspase‑8 and ‑9, as well as inhibition of the ERS pathway.

MATERIALS
Product Number
Brand
Product Description

Supelco
Water, for TOC analysis
Sign Into View Organizational & Contract Pricing
Supelco
Water, suitable for ion chromatography
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, BioPerformance Certified
Sign Into View Organizational & Contract Pricing
Supelco
Water, ACS reagent, for ultratrace analysis
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
E-Toxate Water, endotoxin, free
Sign Into View Organizational & Contract Pricing
Supelco
Density Standard 998 kg/m3, H&D Fitzgerald Ltd. Quality
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, PCR Reagent
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, Nuclease-Free Water, for Molecular Biology
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, for cell biology, sterile ultrafiltered
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, for molecular biology, sterile filtered
Sign Into View Organizational & Contract Pricing
Supelco
Water, for HPCE, for luminescence, suitable for UV/Vis spectroscopy
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, tested according to Ph. Eur.
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, suitable for HPLC
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, HPLC Plus
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, ACS reagent
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Water, Deionized
Sign Into View Organizational & Contract Pricing
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
Sign Into View Organizational & Contract Pricing
Pure Water Density Standard, UKAS ISO/IEC17025 and ISO Guide 34 certified, density: 0.9982 g/mL at 20 °C, density: 0.9970 g/mL at 25 °C
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, 98%
Sign Into View Organizational & Contract Pricing
Sigma-Aldrich
Thiazolyl Blue Tetrazolium Bromide, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
Sign Into View Organizational & Contract Pricing