Skip to Content
MilliporeSigma
  • The HO-1/CO system regulates mitochondrial-capillary density relationships in human skeletal muscle.

The HO-1/CO system regulates mitochondrial-capillary density relationships in human skeletal muscle.

American journal of physiology. Lung cellular and molecular physiology (2015-07-19)
Shelly R H Pecorella, Jennifer V F Potter, Anne D Cherry, Dionne F Peacher, Karen E Welty-Wolf, Richard E Moon, Claude A Piantadosi, Hagir B Suliman
ABSTRACT

The heme oxygenase-1 (HO-1)/carbon monoxide (CO) system induces mitochondrial biogenesis, but its biological impact in human skeletal muscle is uncertain. The enzyme system generates CO, which stimulates mitochondrial proliferation in normal muscle. Here we examined whether CO breathing can be used to produce a coordinated metabolic and vascular response in human skeletal muscle. In 19 healthy subjects, we performed vastus lateralis muscle biopsies and tested one-legged maximal O2 uptake (V̇o2max) before and after breathing air or CO (200 ppm) for 1 h daily for 5 days. In response to CO, there was robust HO-1 induction along with increased mRNA levels for nuclear-encoded mitochondrial transcription factor A (Tfam), cytochrome c, cytochrome oxidase subunit IV (COX IV), and mitochondrial-encoded COX I and NADH dehydrogenase subunit 1 (NDI). CO breathing did not increase V̇o2max (1.96 ± 0.51 pre-CO, 1.87 ± 0.50 post-CO l/min; P = not significant) but did increase muscle citrate synthase, mitochondrial density (139.0 ± 34.9 pre-CO, 219.0 ± 36.2 post-CO; no. of mitochondrial profiles/field), myoglobin content and glucose transporter (GLUT4) protein level and led to GLUT4 localization to the myocyte membrane, all consistent with expansion of the tissue O2 transport system. These responses were attended by increased cluster of differentiation 31 (CD31)-positive muscle capillaries (1.78 ± 0.16 pre-CO, 2.37 ± 0.59 post-CO; capillaries/muscle fiber), implying the enrichment of microvascular O2 reserve. The findings support that induction of the HO-1/CO system by CO not only improves muscle mitochondrial density, but regulates myoglobin content, GLUT4 localization, and capillarity in accordance with current concepts of skeletal muscle plasticity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Water, BioPerformance Certified
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
(±)-Propylene oxide, puriss. p.a., ≥99.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
E-Toxate Water, endotoxin, free
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, PCR Reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, Nuclease-Free Water, for Molecular Biology
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, sterile-filtered, BioReagent, suitable for cell culture
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, for cell biology, sterile ultrafiltered
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, for molecular biology, sterile filtered
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Vinyl laurate, produced by Wacker Chemie AG, Burghausen, Germany, ≥98% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Vinyl laurate, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, ACS reagent
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Water, Deionized
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Osmium tetroxide, Sealed ampule.
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
(±)-Propylene oxide, ReagentPlus®, ≥99%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, 50 wt. % in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaraldehyde solution, SAJ first grade, 20.0-26.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Os EnCat® 40, extent of labeling: 0.3 mmol/g Os loading
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Glutaric dialdehyde solution, 50 wt. % in H2O, FCC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00
Sigma-Aldrich
Osmium tetroxide, ReagentPlus®, 99.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
250 mg
Only 2 left in stock
Details...
$336.00
1 g
In Stock
Details...
$596.00