Skip to Content
MilliporeSigma
  • Investigation of the protective effects of proanthocyanidin and vitamin E against the toxic effect caused by formaldehyde on the liver tissue.

Investigation of the protective effects of proanthocyanidin and vitamin E against the toxic effect caused by formaldehyde on the liver tissue.

Environmental toxicology (2014-06-17)
Elvan Bakar, Enis Ulucam, Aysegul Cerkezkayabekir
ABSTRACT

We aimed to investigate of protective role of proanthocyanidin (PA) and vitamin E (vit E) against to toxic effect of formaldehyde (FA). Twenty-eight Wistar albino rats were divided into four groups: control group, rats treated with FA intraperitoneal (i.p.) (10 mg/kg), FA + vit E intragastric (i.g.) (30 mg/kg), and FA + PA i.g. (100 mg/kg). We assayed superoxide dismutase (SOD), glutathione peroxidase (Gpx), myeloperoxidase (MPO) activity and levels of malondialdehyde (MDA) and total sialic acid (TSA) in liver. Liver tissue was taken in order to morphological analysis and hepatocytes apoptosis using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay immunostaining. SOD decreased in FA and increased in FA + vit E and FA + PA (p < 0.05). Gpx didn't change in FA and increased in FA + PA (p < 0.05). No significant variation between the groups was found in MPO activity. MDA increased only in FA and decreased in FA + vit E and FA+PA (p < 0.05). TSA didn't alter in FA and FA + vit E but decreased in FA + PA (p < 0.05). Degeneration in hepatocytes and endothelial cells, cytoplasm losses, vacuolization, picnotic nuclei, and mononuclear cell infiltration were identified in FA. Degeneration in chromatin material, membrane damage in mitochondria and losses in mitochondrial cristae in hepatocytes were observed in FA. We found that partially recovery in liver as a result of FA + vit E and FA + PA. We have concluded that long term use should be investigated for complete explanation of PA's protective effects on FA toxicity.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium dodecyl sulfate, 92.5-100.5% based on total alkyl sulfate content basis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ≥90% ((Assay))
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ACS reagent, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, tested according to NF, mixture of sodium alkyl sulfates consisting mainly of sodium dodecyl sulfate
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ≥98.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, JIS special grade, 94.8-95.8%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, SAJ special grade, ≥97.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Supelco
Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC), free-flowing, Redi-Dri
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, ReagentPlus®, ≥98.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, BioXtra, ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Sodium dodecyl sulfate, BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethyl alcohol, Pure, 190 proof, for molecular biology
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
o-Xylene, SAJ special grade, ≥98.5%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
o-Xylene, reagent grade, ≥98.0%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
o-Xylene, puriss. p.a., ≥99.0% (GC)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for absorption spectrum analysis
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, HPLC/spectrophotometric grade
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, purum, fine spirit, denaturated with 4.8% methanol, F25 METHYL1, ~96% (based on denaturant-free substance)
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, ACS reagent, prima fine spirit, without additive, F15 o1
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
o-Xylene, anhydrous, 97%
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00
Sigma-Aldrich
Ethanol, ≥99.5%, suitable for HPLC
Sign Into View Organizational & Contract Pricing
SKUPack SizeAvailabilityPriceQuantity
500 g
In Stock
Details...
$162.00
1 kg
In Stock
Details...
$274.00
5 kg
In Stock
Details...
$752.00