Skip to Content
MilliporeSigma

Complexation-triggerable liposome mixed with silk protein and chitosan.

Journal of biomaterials science. Polymer edition (2015-06-11)
Yeon-Ji Hong, Jin-Chul Kim
ABSTRACT

Complexation-triggerable liposomes were prepared by modifying the surface of egg phosphatidylcholine (EPC) liposomes with hydrophobicized silk fibroin (HmSF) and hydrophobicized chitosan (HmCh). Maximum complexation, determined by measuring the diameter of complexation, was found when the ratio of HmSF to HmCh was 14:1, so they were immobilized on the surface of liposomes at the same ratio. The degree of fluorescence quenching of calcein in liposomal suspension was as high as 68% when the ratio of surface modifier (HmSF + HmCh) to EPC was 1:15. When the ratio was increased to 1:5, the degree of quenching decreased to 32%, indicating the inefficient formation of liposome. Liposome mixed with the surface modifier was multi-lamellar vesicle on TEM photo. And, the mean diameter was larger than those of liposome mixed with either HmSF or HmCh, possibly due to insoluble complex on the liposomal surface. The liposome exhibited a pH-sensitive release and triggered the release at pH 5.5 and 6.0. It is believed that complexation is responsible for the promoted release at those pH values.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0% (GC), dust-free pellets
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium dodecyl sulfate, SAJ special grade, ≥97.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium dodecyl sulfate, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, 99.5-100.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, SAJ first grade, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, JIS special grade, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid solution, 1 M, 1 N
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, SAJ special grade
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, NMR reference standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ammonia-14N, 99.99 atom % 14N
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
SKU
Pack Size
Availability
Price
Quantity
Supelco
Sodium dodecyl sulfate, dust-free pellets, suitable for electrophoresis, for molecular biology, ≥99.0% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyrene, puriss. p.a., for fluorescence, ≥99.0% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, 99.93%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid, natural, ≥99.5%, FG
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium dodecyl sulfate, ≥90% ((Assay))
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium dodecyl sulfate, BioUltra, for molecular biology, ≥99.0% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Ammonia, anhydrous, ≥99.98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Sodium dodecyl sulfate, ACS reagent, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyrene, sublimed grade, 99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hexanoic acid, ≥99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Pyrene, 98%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Methanol, anhydrous, 99.8%
SKU
Pack Size
Availability
Price
Quantity