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RP-HPLC-DAD-MS(n) Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts.

RP-HPLC-DAD-MS(n) Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts.

Natural product communications (2015-07-23)

Jósquia S Barbosa, Verônica M Almeida, Rosilene M Marçal, Alexsandro Branco

ABSTRACT

High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3-O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, suitable for HPLC

Sigma-Aldrich

Methanol, JIS 300, ≥99.8%, for residue analysis

Sigma-Aldrich

Methanol, JIS special grade, ≥99.8%

Sigma-Aldrich

Acetic acid, ≥99.7%

Sigma-Aldrich

Acetic acid, ≥99.7%, suitable for amino acid analysis

Sigma-Aldrich

Methanol, SAJ first grade, ≥99.5%

Sigma-Aldrich

Acetic acid solution, 1 M, 1 N

Sigma-Aldrich

Methanol, SAJ special grade

Sigma-Aldrich

Acetic acid, SAJ first grade, ≥99.0%

Sigma-Aldrich

Acetic acid, ≥99.7%

Sigma-Aldrich

Acetic acid, JIS special grade, ≥99.7%

Sigma-Aldrich

Acetic acid, 99.5-100.0%

Sigma-Aldrich

Methanol, NMR reference standard

Sigma-Aldrich

Acetic acid-¹²C₂, 99.9 atom % ¹²C

Sigma-Aldrich

Trizma^® hydrochloride, BioXtra, pH 3.5-5.0 (0.5 M in H₂O), ≥99.0% (titration)

Sigma-Aldrich

5,5′-Dithiobis(2-nitrobenzoic acid), ≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sigma-Aldrich

Trizma^® hydrochloride, BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

Trizma^® hydrochloride, reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

Trizma^® hydrochloride, BioUltra, for molecular biology, ≥99.0% (AT)

Sigma-Aldrich

Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)

Sigma-Aldrich

Acetic acid, natural, ≥99.5%, FG

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, 99.93%

Sigma-Aldrich

Acetic acid, ≥99.5%, FCC, FG

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

5,5′-Dithiobis(2-nitrobenzoic acid), ReagentPlus^®, 99%

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%, poly-coated bottles

Sigma-Aldrich

Trizma^® hydrochloride, anhydrous, free-flowing, Redi-Dri^™, ≥99.0%

Sigma-Aldrich

Ethyl acetate, ReagentPlus^®, ≥99.8%

Sigma-Aldrich

Hexane, anhydrous, 95%

Sigma-Aldrich

Ethyl acetate, natural, ≥99%, FCC, FG