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  • RP-HPLC-DAD-MS(n) Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts.

RP-HPLC-DAD-MS(n) Analysis and Butyrylcholinesterase Inhibitory Activity of Barbacenia blanchetii Extracts.

Natural product communications (2015-07-23)
Jósquia S Barbosa, Verônica M Almeida, Rosilene M Marçal, Alexsandro Branco
ABSTRACT

High-performance liquid chromatography (HPLC) coupled to a diode-array detector (DAD) and electrospray ionization mass spectrometry (MS) was applied for the separation and identification of phenolic compounds in the n-hexane, ethyl acetate and methanol crude extract of Barbacenia blanchetii. The MS, MSn and UV data together with chemosystematic evidence allowed the structural characterization of five compounds: tricin, chrysoeriol, epi-gallocatechin, kaempferol 3-O-glucoside and caffeoylquinic acid. At the same time, these extracts were evaluated against butyrylcholinesterase using Ellman's method. All extracts inhibited BuChE in a concentration-dependent form; however, the methanol extract showed a better effect that the other extracts. These compounds have been identified previously in the Velloziaceae family, but for the first time as constituents of B. blanchetii.

MATERIALS
Product Number
Brand
Product Description

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