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T6666

Sigma-Aldrich

Trizma® hydrochloride

pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

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Synonym(s):
TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein:
3675235
EC Number:
MDL number:
PubChem Substance ID:

product line

BioXtra

Quality Level

Assay

≥99.0% (titration)

form

powder

impurities

Insoluble matter, passes filter test

ign. residue (900 °C)

≤0.2% (as SO4)

loss

≤0.2% loss on drying, 110°C

pH

3.5-5.0 (0.5 M in H2O)

useful pH range

7.0-9.0

pKa (25 °C)

8.1

mp

150-152 °C

solubility

H2O: 0.5 M, clear, colorless

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

absorption

≤0.010 at 280 in H2O at 0.5 M
≤0.015 at 260 in H2O at 0.5 M

application(s)

diagnostic assay manufacturing

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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1 of 4

This Item
T5941T3253RDD009
Trizma® hydrochloride pH 3.5-5.0 (0.5 M in H2O), BioXtra, ≥99.0% (titration)

Sigma-Aldrich

T6666

Trizma® hydrochloride

Premium Grade
Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

T5941

Trizma® hydrochloride

Premium Grade
Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

Essential Grade
Trizma® hydrochloride anhydrous, free-flowing, Redi-Dri™, ≥99.0%

Sigma-Aldrich

RDD009

Trizma® hydrochloride

-
assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99.0% (titration)

assay

≥99.0%

form

powder

form

crystalline powder

form

crystalline

form

powder

impurities

Insoluble matter, passes filter test

impurities

DNAse, Exonuclease; NICKase, Endonuclease; RNAse and Protease, none detected, endotoxin and total aerobic microbial count, tested, ≤0.5% water (Karl Fischer)

impurities

≤0.5% water (Karl Fischer)

impurities

≤0.5% water (Karl Fischer)

pH

3.5-5.0 (0.5 M in H2O)

pH

-

pH

-

pH

-

mp

150-152 °C

mp

150-152 °C

mp

150-152 °C

mp

150-152 °C

Other Notes

Easily compare specifications for Trizma HCl products with the Trizma HCl specification table.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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