MilliporeSigma
All Photos(1)

Documents

T6455

Sigma-Aldrich

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever

Sign Into View Organizational & Contract Pricing

NACRES:
NA.47

biological source

synthetic

Quality Level

pH

10

application(s)

hematology
histology

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
9153873C9999
vibrant-m

915

Citrate Solution

vibrant-m

3873

Tartrate Solution

pH

10

pH

3.6±0.1 (25 °C)

pH

(4.9 +/- 0.1)

pH

6.0

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

application(s)

hematology
histology

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

biological source

synthetic

biological source

-

biological source

-

biological source

-

Application

Tris-HCl Buffer, pH 10, 10×, Antigen Retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. The use of Tris-HCl buffer, pH 10, or other antigen retrieval solutions on FFPE tissue sections improves accessibility of antibodies to tissue antigens.

Preparation Note

Dilute the Tris-HCl Buffer, pH 10, 10×, and Antigen Retriever 10-fold with water to prepare a 1 × Working Solution, e.g., dilute 10 mL of 10 × concentrate with 90 mL of water.

Storage and Stability

Store the product at 2–8 °C. Do Not Freeze to prevent possible precipitation.

Other Notes

Occasionally the buffer may contain a yellowish tinge. This is due to preservative and will not affect the function of the buffer. Store the prepared 1′ Tris-HCl Buffer, pH 10, Working Solution at 2–8 °C.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

Trizma® hydrochloride BioPerformance Certified, suitable for cell culture, ≥99.0% (titration)

Sigma-Aldrich

T5941

Trizma® hydrochloride

Trizma® hydrochloride BioUltra, for molecular biology, ≥99.0% (AT)

Sigma-Aldrich

93363

Trizma® hydrochloride

Trizma® hydrochloride reagent grade, ≥99.0% (titration), crystalline

Sigma-Aldrich

T3253

Trizma® hydrochloride

High expression of stromal PDGFRβ is associated with reduced benefit of tamoxifen in breast cancer.
Paulsson J, et al.
The journal of pathology. Clinical research, 3(1), 38-43 (2017)
S R Shi et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 43(2), 193-201 (1995-02-01)
Antigen retrieval (AR) incorporating high-temperature microwave (MW) heating of tissue sections before immunostaining is a revolutionary technique that can unmask the antigens in formalin-fixed tissue sections, thus making them available for immunohistochemical staining. Although high temperature is believed to be
Hilde Ytre-Hauge Smeland et al.
BMC cancer, 19(1), 234-234 (2019-03-17)
Cancer progression is influenced by a pro-tumorigenic microenvironment. The aberrant tumor stroma with increased collagen deposition, contractile fibroblasts and dysfunctional vessels has a major impact on the interstitial fluid pressure (PIF) in most solid tumors. An increased tumor PIF is

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service