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SBR00051

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.5, DNase and Microbial DNA free, 10 mM

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Synonym(s):
Trizma® hydrochloride, TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride, Tromethane hydrochloride
Linear Formula:
NH2C(CH2OH)3 · HCl
CAS Number:
Molecular Weight:
157.60
Beilstein/REAXYS Number:
3675235
MDL number:

Quality Level

concentration

10 mM

pH

8.5

useful pH range

7.0-9.0

pKa (25 °C)

8.1

storage temp.

2-8°C

SMILES string

Cl.NC(CO)(CO)CO

InChI

1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H

InChI key

QKNYBSVHEMOAJP-UHFFFAOYSA-N

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This Item
T15760T325393363
vibrant-m

SBR00051

Trizma® hydrochloride solution

-
vibrant-m

T15760

Trizma® hydrochloride

Essential Grade
vibrant-m

T3253

Trizma® hydrochloride

Essential Grade
vibrant-m

93363

Trizma® hydrochloride

Premium Grade
concentration

10 mM

concentration

-

concentration

-

concentration

-

pH

8.5

pH

-

pH

-

pH

2.5-4.0 (25 °C, 4 M in H2O)

storage temp.

2-8°C

storage temp.

-

storage temp.

-

storage temp.

-

useful pH range

7.0-9.0

useful pH range

7.0-9.0

useful pH range

7.0-9.0

useful pH range

7.0-9.0

pKa (25 °C)

8.1

pKa (25 °C)

8.1

pKa (25 °C)

8.1

pKa (25 °C)

8.1

General description

10 mM Trizma® hydrochloride (pH 8.5) is a widely used buffer in molecular biology applications such as nucleic acid extraction and purification. No mixing or pH adjustment is necessary. Guaranteed accuracy for this buffer is ± 0.2 pH units at 25°C.

Application

Trizma® hydrochloride solution can be used:
  • As an elution buffer after PCR cleanup
  • As an elution buffer after DNA extraction

Preparation Note

Allow the buffer to reach room temperature before use.

Other Notes

Prepared with BioUltra Trizma Base for molecular biology in 18 megaohm UV irradiated water. pH-adjusted and 0.2 μm filtered. The buffer was tested by 35 cycles of PCR for prokaryotic and eukaryotic DNA contamination. To keep the buffer contamination-free, it is recommended to use gloves and barrier-tip pipettes for dispensing. Between uses, keep the bottle closed and refrigerated, away from areas where DNA samples and PCR products may be stored.
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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