Skip to Content
MilliporeSigma
  • Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells.

Silk fibroin scaffolds enhance cell commitment of adult rat cardiac progenitor cells.

Journal of tissue engineering and regenerative medicine (2013-04-18)
Valentina Di Felice, Claudia Serradifalco, Luigi Rizzuto, Angela De Luca, Francesca Rappa, Rosario Barone, Patrizia Di Marco, Giovanni Cassata, Roberto Puleio, Lucia Verin, Antonella Motta, Claudio Migliaresi, Annalisa Guercio, Giovanni Zummo
ABSTRACT

The use of three-dimensional (3D) cultures may induce cardiac progenitor cells to synthesize their own extracellular matrix (ECM) and sarcomeric proteins to initiate cardiac differentiation. 3D cultures grown on synthetic scaffolds may favour the implantation and survival of stem cells for cell therapy when pharmacological therapies are not efficient in curing cardiovascular diseases and when organ transplantation remains the only treatment able to rescue the patient's life. Silk fibroin-based scaffolds may be used to increase cell affinity to biomaterials and may be chemically modified to improve cell adhesion. In the present study, porous, partially orientated and electrospun nanometric nets were used. Cardiac progenitor cells isolated from adult rats were seeded by capillarity in the 3D structures and cultured inside inserts for 21 days. Under this condition, the cells expressed a high level of sarcomeric and cardiac proteins and synthesized a great quantity of ECM. In particular, partially orientated scaffolds induced the synthesis of titin, which is a fundamental protein in sarcomere assembly.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
Sigma-Aldrich
Osmium tetroxide, ReagentPlus®, 99.8%
Sigma-Aldrich
Osmium tetroxide, ACS reagent, ≥98.0%
Sigma-Aldrich
Methanol, BioReagent, ≥99.93%
Sigma-Aldrich
Lithium bromide solution, 54 wt. % in H2O
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Acetone, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Ethanol, JIS first grade, 94.8-95.8%
Sigma-Aldrich
Acetone, for residue analysis, JIS 5000
Sigma-Aldrich
Methanol, suitable for HPLC
Sigma-Aldrich
Acetone, for chromatography, ≥99.8%
Sigma-Aldrich
Acetone, suitable for HPLC
Sigma-Aldrich
Acetone, SAJ first grade, ≥99.0%
Sigma-Aldrich
Acetone, JIS special grade, ≥99.5%
Sigma-Aldrich
Acetone, ≥99.5%, for residue analysis
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Acetone, for residue analysis, ≥99.5%
Sigma-Aldrich
Os EnCat® 40, extent of labeling: 0.3 mmol/g Os loading
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Methanol, NMR reference standard
Supelco
Ethanol solution, certified reference material, 2000 μg/mL in methanol
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Ethanol Fixative 80% v/v, suitable for fixing solution (blood films)
Sigma-Aldrich
Trypsin-EDTA solution, 10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride
Sigma-Aldrich
Glutaraldehyde solution, Grade II, 25% in H2O
Sigma-Aldrich
Glutaraldehyde solution, 50% in H2O, suitable for photographic applications