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  • Multiple pathways facilitate the biogenesis of mammalian tail-anchored proteins.

Multiple pathways facilitate the biogenesis of mammalian tail-anchored proteins.

Journal of cell science (2017-10-13)
Joseph Casson, Michael McKenna, Sarah Haßdenteufel, Naama Aviram, Richard Zimmerman, Stephen High
ABSTRACT

Tail-anchored (TA) proteins are transmembrane proteins with a single C-terminal transmembrane domain, which functions as both their subcellular targeting signal and membrane anchor. We show that knockout of TRC40 in cultured human cells has a relatively minor effect on endogenous TA proteins, despite their apparent reliance on this pathway

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Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
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L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
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Casein Blocking Buffer 10x, for Northern and Southern blotting, solution