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HPLC Analysis of Phenylephrine Enantiomers on Astec® CYCLOBOND I 2000 AC

HPLC Analysis of Phenylephrine Enantiomers on Astec® CYCLOBOND™ I 2000 AC application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Astec® CYCLOBOND I 2000 AC Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

(R)-(−)-Phenylephrine hydrochloride

analytical standard

(R)-(−)-Phenylephrine hydrochloride

powder

(R)-(−)-Phenylephrine hydrochloride

tested according to Ph. Eur.

CONDITIONS

column

CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)

mobile phase

[A] 0.5% w/v sodium acetate, pH 5.5; [B] methanol; (90:10, A:B)

flow rate

0.5 mL/min

column temp.

22 °C

detector

UV, 254 nm

injection

1 μL

sample

1 mg/mL in methanol

Description

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CYCLOBOND is a trademark of Sigma-Aldrich Co. LLC