HPLC Analysis of Protein Standards on Zenix^® SEC-300 in the Presence of a Denaturant

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

[A] 0.1 M ammonium bicarbonate (pH unadjusted); [B] 0.1 M ammonium bicarbonate (pH unadjusted) with 6 M guandine hydrochloride (pH unadjusted)

flow rate

0.3 mL/min

pressure

1300 psi (90 bar)

column temp.

25 °C

detector

UV, 280 nm

injection

2.5 μL

sample

Thyroglogulin, 1.5 g/L; IgG, 1 g/L; Albumin, 2.5 g/L; Myoglobin, 1 g/L; Uracil, 0.05 g/L; in mobile phase