HPLC Analysis of Proteins on BIOshell A160 Peptide C18

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A