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HPLC Analysis of Proteins on BIOshell A400 Protein C18

HPLC Analysis of Proteins on BIOshell A400 Protein C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

BIOshell A400 Protein C18 Column

L × I.D. 10 cm × 2.1 mm, 3.4 μm particle size

Standard

Product No.
Description
Pricing

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

Albumin from chicken egg white

For use as a marker in SDS-PAGE

Lysozyme from chicken egg white

lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

Conalbumin from chicken egg white

Substantially iron-free

Haptoglobin Human, Phenotype 1-1

98-100%, essentially salt-free, lyophilized powder

Trypsin Inhibitor from Glycine max (soybean)

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

CONDITIONS

column

BIOShell A400 C18, 10 cm x 2.1 mm I.D., 3.4 μm particles (67463-U)

mobile phase

[A] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 75:25; [B] (water, 0.1% TFA); (acetonitrile, 0.08% TFA), 25:75

gradient

20 to 100% B in 8 min

flow rate

0.3 mL/min

pressure

2480 psi total pressure (193 bar)

column temp.

30 °C

detector

UV, 215 nm

injection

2 μL

sample

lysozyme, 0.06; haptoglobulin, 0.06; conalbumin, 0.12; tryspin inhibitor, 0.18; glutamate dehydrogenase, (0.18; ß-galactosidase, 0.12; serum albumin chicken, 0.12 g/L in mobile phase A)

Description

Analysis Note

Analysis of proteins of various hydrophobicity on BIOshell A400 Protein C18 to compare the selectivity and retention times.

Legal Information

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