HPLC Analysis of Steroids on Ascentis® Express Biphenyl
Materials
analytical column
Product No.
Description
Pricing
standard
Product No.
Description
Pricing
Corticosterone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Estriol solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®Progesterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Cortisone solution
100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express Biphenyl, 5 cm x 4.6 mm I.D., 2.7 μm particles (64059-U)
mobile phase
[A] water; [B] acetonitrile
gradient
20 to 60% B in 6 min
flow rate
1.85 mL/min
pressure
4998 psi (344 bar)
column temp.
30 °C
detector
UV, 215 nm
injection
4 μL
sample
25 ng/mL each except 125 ng/mL deoxycorticosterone in 62.5:37.5, water:acetonitrile
Description
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany