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HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

50538-U

Ascentis® Express C18, 5 μm HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

E5750

Ergocalciferol

40,000,000 USP units/g

C9756

Cholecalciferol

≥98% (HPLC)

CONDITIONS

column

Ascentis® Express C18, 25 cm x 4.6 mm I.D., 5 μm particles (50538-U)

mobile phase

[A] acetonitrile; [B] methanol; (95:5, A:B)

flow rate

1.5 mL/min

pressure

1726 psi (119 bar)

column temp.

35 °C

detector

UV, 265 nm

injection

10 μL

sample

50 μg/mL in 90:10, acetonitrile:methanol

Description

Analysis Note

This application demonstrates the suitability of the Ascentis Express C18, 5 μm, for the routine analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis Express C18, 5 μm are presented.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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