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HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column application for HPLC

Materials

analytical column

Product No.
Description
Pricing

TSKgel® BioAssist® S HPLC Column

phase sulfonic acid, L × I.D. 5 cm × 4.6 mm, 7 μm particle size, PEEK hardware

TSKgel® BioAssist® S HPLC Column

phase sulfonic acid, L × I.D. 10 cm × 10 mm, 13 μm particle size, PEEK hardware

mobile phase component

Product No.
Description
Pricing

Sodium chloride

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

standard

Product No.
Description
Pricing

Bromelain from pineapple stem

≥3 units/mg protein

CONDITIONS

column

TSKgel Bio Assist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)

column

Competitive S-type column, 5 cm x 5 mm I.D.

mobile phase

[A] 20 mM sodium phophate buffer, pH 7.0; [B] 20 mM sodium phophate buffer, 0.5 M NaCl, pH 7.0

gradient

0 to 100% B in 20 min (0 to 100% B in 30 min for competitive column)

flow rate

0.8 mL/min (1.0 mL/min for competitive column)

column temp.

25 °C

detector

UV, 280 nm

sample

crude bromelain, 0.01 μg/mL

Description

Other Notes

Data provided by Tosoh Bioscience LLC.

Legal Information

BioAssist is a registered trademark of Tosoh Corporation
TSKgel is a registered trademark of Tosoh Corporation