Astec^® CHIROBIOTIC^® Chiral HPLC Columns

Macrocyclic Glycoprotein Stationary Phases for Chiral Separations and Highly Selective Achiral Separations

CHIROBIOTIC^® phases are based on covalently bonding macrocyclic glycoproteins to a high-purity 5-micron silica gel in such a way as to establish its stability while retaining essential components for chiral recognition. CHIROBIOTIC^® V and V2 are based on bonding Vancomycin, which contains 18 chiral centers surrounding three pockets or cavities. Five aromatic ring structures bridge these strategic cavities. Hydrogen donor-acceptor sites are readily available close to the ring structures. CHIROBIOTIC^® V has demonstrated selectivity similar to glycoprotein phases except it is stable from 0-100% organic modifier and exhibits high sample capacity.

For CHIROBIOTIC^® V2, changes to the linkage chemistry and silica offer improvements for preparative LC and for more demanding chiral separations. CHIROBIOTIC^® T, T2, and TAG are based on bonding the amphoteric glycopeptide, Teicoplanin, which contains 23 chiral centers surrounding four pockets or cavities. For CHIROBIOTIC^® T2, changes to the linkage chemistry and silica offer improvements for preparative LC and for more demanding chiral separations. CHIROBIOTIC^® TAG has the sugars removed from the macrocyclic glycopeptide to produce an aglycone structure as a variant of CHIROBIOTIC^® T. CHIROBIOTIC^® R is based on bonding Ristocetin A to high purity 5-micron silica.