These products are available in the US, Canada, and select European countries.
Osteoblasts are a highly specialized cells that originate from the mesenchyme. Human osteoblasts play an important role in bone formation and remodeling. In vivo, they produce the osteoid, an extracellular matrix-rich in collagen type I. During osteogenesis, the natural maturation process into osteocytes, they embed in the bone matrix and stop proliferating.
PromoCell® Human Osteoblasts (HOB) are produced from knee and hip femoral tissue. Shortly after isolation, all Human Osteoblasts are cryopreserved at passage 2 (P2) using proprietary, serum-free freezing medium, Cryo-SFM. Each thawed cryovial contains at least 500,000 viable cells. Human Osteoblasts are also available as proliferating cell cultures generated from cryopreserved cells that have been thawed and then cultured for three days.
The Osteoblast Growth Medium is optimized for culturing human osteoblasts in vitro and the Osteoblast Mineralization Medium was developed to induce robust mineralization in human osteoblast cultures with a one-step protocol, which leads to extensive extracellular calcium deposits after 21 days. These media are optimized for growing primary human cells, but can also be used for bovine, murine, and rat osteoblasts as well as osteoblast cell lines. The Osteoblast Media (ready-to-use) consists of one 500 mL bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium constitutes the complete Osteoblast Media. The Osteoblast Basal Medium (with or without phenol red) and Osteoblast Growth Medium SupplementMix are also available for separate purchase.
The Osteoblast Growth Medium contains all the necessary components for the optimal growth of human osteoblasts. The Osteoblast Mineralization Medium contains all the components necessary for mineralization of human osteoblasts. Osteoblast Media do not contain antibiotics or antimycotics and are formulated for use in an incubator with 5 % atmospheric CO2.
The following rigid quality control tests are performed for each lot of Human Osteoblasts:
A detailed certificate of analysis (CoA) for each lot is available for download.
Human Osteoblast Specifications
All lots of Osteoblast Growth Medium are subjected to comprehensive quality control testing. These tests check for growth-promoting activity, adherence rate, and typical cell morphology using primary human osteoblasts. Further, all lots of Osteoblast Mineralization Medium are tested for their capacity to induce mineralization in primary human osteoblasts. Approved in-house lots of Osteoblast media are used as a reference. Additionally, microbial testing ensures that all lots of Osteoblast Growth/Differentiation Media are free of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Human Primary Osteoblasts and Osteoblast Growth/Differentiation Media
Human Osteoblasts and Growth/Differentiation Media are intended for in vitro use only and not for diagnostic or therapeutic procedures. For safety information please see appropriate MSDS.
Note: PromoCell media have low- or zero-serum content and are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead, we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
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