These products are available in the US, Canada, and select European countries.
Connective tissue is derived from the mesoderm and is critical for maintaining the structural integrity of the body. Fibroblasts, the predominant cells found in connective tissue, continuously secrete diverse components of the extracellular matrix. Fibroblasts exhibit unique morphology and functional properties that depend on their site of origin and physiological status.
Primary Human Fibroblasts from PromoCell are isolated from normal human juvenile foreskin or from adult skin from various sites, including adult lung tissue and aortic adventitial tissue. Soon after isolation, Human Fibroblasts are cryopreserved at passage 2 (P2) using proprietary, serum-free freezing medium, Cryo-SFM. Each thawed cryovial contains at least 500,000 viable cells. Human Fibroblasts are also available as proliferating cell cultures made from cryopreserved cells that have been thawed and cultured for three days.
Fibroblast Growth Media is available in three different formulations:
All Fibroblast Growth Media formulations are available in two options: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option includes a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit includes a 500 mL bottle of Basal Medium and the SupplementPack, which is a set of individual vials with pre-measured supplements, giving the user full control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium generates the complete Fibroblast Growth Media. The Fibroblast Basal Medium (with or without phenol red), SupplementMix, and SupplementPack may also be purchased separately.
The Fibroblast Growth Media includes all the components necessary for the optimally growing human fibroblasts (for details see table below). Fibroblast Growth Media does not contain antibiotics or antimycotics and is formulated for use in an incubator with 5% atmospheric CO2.
Human Fibroblasts Quality Control
The following rigid quality control tests are performed on each lot of Human Fibroblasts:
*catalog numbers in this table are for media in ready-to-use packaging
All lots of Fibroblast Growth Media are subjected to comprehensive quality control testing. These tests check for growth promoting activity, adherence rate, and typical morphology of primary Human Fibroblasts. Approved in-house lots of media are used as a reference. Additionally, microbial testing ensures that all lots of Fibroblast Growth Media are free of contaminants (fungi, bacteria, mycoplasma).
Primary Human Fibroblasts and Growth Media are intended for in vitro use only and not for diagnostic or therapeutic procedures. For safety information please see appropriate MSDS.
Note: PromoCell media have low or zero serum content and are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead we recommend using the DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
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