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C-12375

Sigma-Aldrich

Human Cardiac Fibroblasts (HCF)

500,000 cryopreserved cells

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Synonym(s):
HCF cells

biological source

human heart (ventricles)

packaging

pkg of 500,000 cells

morphology

(fibroblast)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

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This Item
C-12810C-12302C-14080
packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

morphology

(fibroblast)

morphology

(myocytes)

morphology

(fibroblast)

morphology

(myocytes)

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

relevant disease(s)

cardiovascular diseases

relevant disease(s)

-

relevant disease(s)

-

relevant disease(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Heart

Application

Primary Human Cardiac Fibroblasts (HCF) are isolated from the ventricles of the adult heart and are provided in a cryopreserved format. They play a central role in the maintenance of the extracellular matrix in the normal heart and the synthesis of growth factors and cytokines. Under pathophysiological conditions, cardiac fibroblasts are involved in scar formation after myocardial infarction, cardiac fibrosis, and cardiac hypertrophy. HCF can be used to study such processes in vitro.

Quality

Rigid quality control tests are performed for each lot of Human Fibroblasts. They are tested for cell morphology, adherence rate and cell viability. Flow cytometric analyses of the fibroblast specific antigen CD90 are performed for each lot. Growth performance is tested through multiple passages up to 10 or 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: CD90 positive, Smooth muscle alpha-actin negative, slow muscle myosin negativeGuaranteed population doublings: >15

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Primary Cell Culture Basics

What are primary cells? A comprehensive overview of animal and human primary cell lines and media and how they are used in biomedical cell culture applications. Primary cultures are less homogeneous relative to cell lines.

Primary Human Fibroblasts and Growth Media

Primary human fibroblasts and growth medium for the in vitro cultivation of juvenile and adult fibroblasts. Protocols for handling, cell passaging, media, and product use.

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