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C-12285

Sigma-Aldrich

Human Cardiac Microvascular Endothelial Cells (HCMEC)

500,000 cryopreserved cells

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biological source

human heart (ventricles)

packaging

pkg of 500,000 cells

morphology

( endothelial)

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

diabetes; hypertension; ischemia/reperfusion injury

shipped in

dry ice

storage temp.

−196°C

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This Item
C-14029C-14027C-14015
packaging

pkg of 500,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

packaging

pkg of 1,000,000 cells

morphology

( endothelial)

morphology

(endothelial)

morphology

(endothelial)

morphology

(endothelial)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

relevant disease(s)

diabetes; hypertension; ischemia/reperfusion injury

relevant disease(s)

diabetes; hypertension; ischemia/reperfusion injury

relevant disease(s)

-

relevant disease(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Heart

Application

Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor and are provided in a cryopreserved format. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin. HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other microvascular endothelial cells. HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.

Quality

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested forthe absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Emma L Wilkinson et al.
Biology open, 5(10), 1362-1370 (2016-08-21)
Cardiotoxicity induced by anti-cancer therapeutics is a severe, and potentially fatal, adverse reaction of the heart in response to certain drugs. Current in vitro approaches to assess cardiotoxicity have focused on analysing cardiomyocytes. More recently it has become apparent that
Matthew Alonzo et al.
ACS applied materials & interfaces, 14(19), 21800-21813 (2022-05-10)
In this study, we developed three-dimensional (3D) printed annular ring-like scaffolds of hydrogel (gelatin-alginate) constructs encapsulated with a mixture of human cardiac AC16 cardiomyocytes (CMs), fibroblasts (CFs), and microvascular endothelial cells (ECs) as cardiac organoid models in preparation for investigating

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