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HL-1 Cardiac Muscle Cell Line

HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.

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HL-1 Cardiomyocyte Cell Line, HL-1 Atrial Muscle Cell Line

biological source


Quality Level


cell based assay: suitable
cell culture | mammalian: suitable

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cell based assay: suitable, cell culture | mammalian: suitable


cell based assay: suitable, cell culture | mammalian: suitable


cell culture | mammalian: suitable


cell culture | mammalian: suitable

Quality Level


Quality Level


Quality Level


Quality Level


General description

HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

Cell Line Description

Cardiomyocyte Cells


Research Category

Cardiovascular Disease

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact


• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium

Storage and Stability

HL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Storage Class Code

10 - Combustible liquids



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Proceedings of the National Academy of Sciences of the United States of America, 95(6), 2979-2984 (1998-04-18)
We have derived a cardiac muscle cell line, designated HL-1, from the AT-1 mouse atrial cardiomyocyte tumor lineage. HL-1 cells can be serially passaged, yet they maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties.
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