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51800C

Sigma-Aldrich

Claycomb Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
HL-1 Medium

description

For research or for further manufacturing use.

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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This Item
51417C51415CW4128
vibrant-m

51800C

Claycomb Medium

vibrant-m

51417C

Minimum Essential Medium

vibrant-m

51415C

Minimum Essential Medium

vibrant-m

W4128

Williams′ Medium E

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

components

L-glutamine: 292 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes
NaHCO3: 840 mg/L
HEPES: 4766.4 mg/L

components

Earle’s salts (5% CO2): yes
HEPES: 25 mM
L-glutamine: no

components

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

Quality Level

500

Quality Level

400

Quality Level

400

Quality Level

400

storage condition

protect from light

storage condition

-

storage condition

-

storage condition

-

Application

Claycomb Medium, named after Dr. William Claycomb who established the HL-1 cell line, is specifically designed for the growth of murine cardiomyocytes. HL-1 is the first cell line established that can maintain the differentiated cardiomyocyte phenotype and contractile activity in vitro. The HL-1 cell line can be used for the study of cardiac cell hypertrophy that follows myocardial infarction, the testing of novel cardiac therapeutic drugs and treatments, the production of high levels of cardiac proteins and the study of mature cardiomyocyte specific genes. Claycomb Medium, when supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine, will maintain the HL-1 cell line and the mature cardiomycyte behavior. While observing the HL-1 cells under light microscopy, individual and groups of cells can be observed contracting, becoming more frequent as the cardiomyocytes reach confluency.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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