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Genomic DNA Prep & Deconvolution Services

Easily Identify Candidate Genes From Pooled Screens

Need help with samples from genomic DNA isolation, NGS library prep, sequencing, and analysis? Our service can provide the results you need for your shRNA, CRISPRi/a/n, or ORF pooled screens.

Why Choose Us as your Deconvolution Partner?

  • Quick turnaround of data (~3-6 weeks) depending on project size
  • Consistency in DNA preparation and sequencing
  • Cost-effective multiplexing of samples - only pay for the data you need
  • Experience with shRNA, CRISPRi/a/n, and ORF pool deconvolution
  • Data provided in an easy-to-analyze format

Use the form below to guide the deconvolution discussion with your representative.

Deconvolution Sample Submission Worksheet

How it works:

Pooled Screening Approach to Identify Modulators of a Pathway

When you use one of our pooled shRNA, CRISPR KO, CRISPRi, CRISPRa, or ORF screening libraries, save money by bundling in deconvolution and we can help you identify the candidate genes that emerge from your screen.

Find our complete range of screens here

DNA Preparation and Deconvolution Workflow

Once you provide us with cell or tumor samples post screening, we will extract genomic DNA, construct NGS libraries, perform sequencing and provide raw counts for the presence of each clone on a per sample basis. We can serve your entire pooled shRNA, CRISPR KO, CRISPRi, CRISPRa, or ORF screening workflow.

DNA preparation and deconvolution workflow for RNAi, ORF and CRISPR screens

Why Use Next-Gen Sequencing Over Other Methods for Pooled Screen Deconvolution?

  • High dynamic range and sensitivity
  • Look for enriched or reduced shRNA, CRISPRi/a/n ORF clones
  • A precise count of shRNA,CRISPRi/a/n ORF clone frequency
  • Analyze multiple pools simultaneously
  • Conducted on custom and genome-wide pools

Find our full range of screens here

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