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Detection and Identification of Campylobacter

Detection, identification, differentiation and cultivation of Campylobacter

The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis.1,2 However, other species, like C. fetus, which causes spontaneous abortions, have also been associated with human illness.2–6
Campylobacter are Gram-negative, spiral-shaped, microaerophilic and motile bacteria with uni- or bi-polar flagella (Figures 1, 2 and 3).

Scanning electron microscope

Figure 1.Scanning electron microscope image: shows the characteristic spiral, or corkscrew, shape of C. jejuni cells. Photo by De Wood; digital colorization by Chris Pooley, USDA/ARS

Scanning electron micrograph

Figure 2.Scanning electron micrograph of the single polar flagellum and corkscrew shape of C. jejuni. These morphologic characteristics contribute to the characteristic darting motility of C. jejuni in the viscous mucous layer of the intestinal lumen. Sean F. Altekruse National Cancer Institute, Rockville.

Electron microscope image of Campylobacters

Figure 3.Electron microscope image of Campylobacters by Jochen Reetz, Bundesinstitut für Risikobewertung (BfR), Germany

The size of a cell is roughly 0.2–0.8 x 0.5–5 µm and, in culture, they can undergo morphological change from spiral to spherical. Most species are catalase- and oxidase-positive,7 with the exception of the catalase-negative C. sputorumC. concisusC. mucosalis and C. helveticus. The metabolism of Campylobacter is chemoorganotrophic, with amino acids and intermediates of the citric acid cycle serving as energy sources; typical carbohydrates cannot be used. Campylobacter reduce nitrate to nitrite, obtaining oxygen for their metabolism by this pathway. These distinctive metabolic reactions can be used for the differentiation and identification of Campylobacter species (Table 1). Commercially available tests are listed in Tables 2 and 3.

Table of differentiating characteristics of Campylobacter

Table 1.Table of differentiating characteristics of Campylobacter species and subspecies

Table 2.Tests for identification and differentiation of Campylobacter
Table 3.Gram staining kits and single solutions

Campylobacter are generally fastidious microorganisms and grow only on complex media that have been amended with diverse essential amino acids and supplements, such as pyruvate, a-ketoglutarate, hemin, formate and other essential ions. For selective isolation of Campylobacter, the growth media can be supplemented with antibiotics like cefoperazone, vancomycin, trimethoprim, amphotericin, cycloheximide, rifampicin, cefsulodin and polymyxin B sulfate. We offer a broad range of specific agars and broths for the detection, identification, differentiation, enumeration and cultivation of Campylobacter (Table 4).

Table 4.Media for Campylobacter (detection, differentiation, and identification )

More details about our products for analytical microbiology.

Table 5.Scientific classification of Campylobacter
Materials
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References

1.
Campylobacter jejuni Current Status and Future Trends. Nachamkin, I., Blaser, M. J., Tompkins, L. S., eds., American Society for Microbiology: Washington, DC (1992).
2.
Tauxe, R. V., Hargrett-Bean, N., Patton, C. M., Wachsmuth, I. K., Campylobacter isolates in the United States, 1982 – 86. Morbid. Mortal. Weekly Rep. (1988), 37, 1–13.
3.
Butzler, J. P., Campylobacter Infection in Man and Animals. CRC Press: Boca Raton, FL (1984)..
4.
Linton D, Owen R, Stanley J. 1996. Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals. Research in Microbiology. 147(9):707-718. https://doi.org/10.1016/s0923-2508(97)85118-2
5.
Patton CM, Shaffer N, Edmonds P, Barrett TJ, Lambert MA, Baker C, Perlman DM, Brenner DJ. 1989. Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States.. 27(1):66-73. https://doi.org/10.1128/jcm.27.1.66-73.1989
6.
Tee W, Anderson BN, Ross BC, Dwyer B. 1987. Atypical campylobacters associated with gastroenteritis.. 25(7):1248-1252. https://doi.org/10.1128/jcm.25.7.1248-1252.1987
7.
Ryan, K. J. and Ray C. G., Eds., Sherris Medical Microbiology, 4th ed., McGraw-Hill: New York, NY (2004), 378 – 80.
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