T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• Synonym(s): Tris-EDTA wash buffer, TE buffer solution
• MDL number: MFCD00236359
• UNSPSC Code: 41105319
• PubChem Substance ID: 329826904
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 200
• sterility: 0.2 μm filtered
• form: liquid
• pH: 7.8-8.2 (1 × in water)
• application(s): sample preparation
• foreign activity: DNase, RNase, none detected
• storage temp.: room temp
• SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

Description

General description

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Application

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]
• gel electrophoresis, including Northern and Southern blotting[3]
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]
• antigen retrieval for immunohistochemistry[3]

Preparation Note

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Other Notes

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Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable