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UC0E01

Sigma-Aldrich

UCOE® Single Expression Puromycin Vector Set

別名:

UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element , UCOE01

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W302406-SAMPLE-K
₩44,051
1 KG
₩155,498
10 KG
₩336,168
25 KG
₩698,422

₩44,051


在庫状況についてはカスタマーサービスにお問い合わせください


サイズを選択してください

表示を変更する
W302406-SAMPLE-K
₩44,051
1 KG
₩155,498
10 KG
₩336,168
25 KG
₩698,422

About This Item

UNSPSCコード:
41106621
eCl@ss:
34360190
NACRES:
NA.51

₩44,051


在庫状況についてはカスタマーサービスにお問い合わせください

輸送温度

dry ice

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1 of 4

当該品目
6143165.060715.32583
TAK-242 InSolution, ≥98%, 25 mM in DMSO, TLR4 inhibitor

5.08336

TAK-242

TLR4 Inhibitor, TAK-242 TAK-242; CAS 243984-11-4; is a cell-permeable compound that selectively binds to Cys747 of TLR4 and selectively disrupts its interaction with adaptor molecules TIRAP and TRAM.

614316

TLR4 Inhibitor, TAK-242

I-BET InSolution, ≥98%, 50 mM in DMSO, A cell-permeable benzodiazepine compound

5.06071

I-BET

form

liquid

form

solid

form

liquid

form

powder

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

storage temp.

−70°C

storage temp.

2-8°C

storage temp.

−70°C

storage temp.

2-8°C

storage condition

OK to freeze, desiccated (hygroscopic), avoid repeated freeze/thaw cycles, protect from light

storage condition

OK to freeze, protect from light

storage condition

OK to freeze, avoid repeated freeze/thaw cycles, desiccated (hygroscopic), protect from light

storage condition

OK to freeze, protect from light

詳細

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HS-puro-SceI is a mammalian expression plasmid vector containing the mouse Rps3 UCOE upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AS-puro-SceI is a mammalian expression plasmid vector containing the murine Rps3 UCOE upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HS-puro-SceI and CET 1019 AS-puro-SceI vectors possess the following features:
  • A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzymes to permit easy insertion of any gene of interest (cDNA or genomic)
  • A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
  • A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
  • A β-lactamase gene for plasmid selection and propagation in the transformed prokaryotic cells in the presence of ampicillin
  • An I-Scel homing restriction endonuclease site for linearization of constructs prior to the transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes


THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

アプリケーション

Research Category
一般的なセルバイオロジー
Research Sub Category
All
Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing.

構成

1) 10ug CET 1019 HS-puro-SceI (+) Vector (CS221296)

2) 10ug CET 1019 AS-puro-SceI (+) Vector (CS221284)

3) 10ug SC HS-puro (-) Vector (CS221286)

4) 10ug SC AS-puro (-) Vector (CS221287)

保管および安定性

Plasmids should be stored at -20C. Avoid repeated freeze-thaw events.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

保管分類コード

10 - Combustible liquids


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

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試験成績書(COA)

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文書ライブラリにアクセスする

Fang Zhang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(9), 1640-1649 (2010-07-01)
DNA methylation may restrict the activity of gene transfer vectors due to inadvertent silencing. In P19 embryonic carcinoma cells in vitro, we found that transgene expression regulated by the SFFV LTR and EF1 alpha promoter declined rapidly within 16 days
Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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