05-1050-M

4G10^® Platinum, Anti-Phosphotyrosine Antibody (mouse monoclonal cocktail IgG2b)

clone 4G10®, Upstate^®, from mouse

• 別名: Anti-phosphotyrosine antibody
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 100
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 4G10®, monoclonal
• 化学種の反応性（ホモロジーによる予測）: all
• メーカー／製品名: Upstate^®
• テクニック: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG2b
• ターゲットの翻訳後修飾: phosphorylation (pTyr)

詳細

詳細

The development of the anti-phosphotyrosine, clone 4G10 in 1989 was a monumental discovery for researchers. 4G10 was the first and is the best single monoclonal antibody for the detection tyrosine phosphorylation. 4G10 is well known for its sensitivity and its ability to detect multiple tyrosine phosphorylations on numerous substrates. It has been validated by thousands of scientific and medical researchers in virtually every application and tyrosine target over the past 2 decades. To improve on something that hundred have tried and no one has succeeded, we pooled 4G10 with the next most highly regarded anti-phosphotyrosine, clone PY20 to make 4G10 Platinum. PY20 itself is a very poor substitute for 4G10, but its additive effect allow for a greater level of detection on more substrates that even 4G10 alone was not capable of.

特異性

Tyrosine-phosphorylated proteins from all species

免疫原

4G10 used Phosphotyramine coupled to KLH and PY20 used phosphotyrosine conjugated to carrier protein

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
一般的な翻訳後修飾

4G10 Platinum, Anti-Phosphotyrosine (mouse monoclonal cocktail IgG2b) detects levels of Phosphotyrosine proteins & has been published & validated for use in WB, ICC, ELISA, IHC, IF, Flow & IP.

Immunoprecipitation: 2-4 μL of a previous lot can immunoprecipitate quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.

品質

Evaluated by Western Blot on a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

Western Blot Analysis: 1:1,000-1:2,000 of this antibody detected tyrosinephosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells.

ターゲットの説明

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

物理的形状

Protein G-Sepharose Chromatography

Format: Purified

Proprietary mixture of protein G purified mouse monoclonals 4G10 (IgG2b) and PY20 (IgG2b) in 0.1 M Tris-glycine, Ph 7.4, 0.15 M NaCl, 0.05% sodium azide.
Liquid at 2-8°C.

保管および安定性

Stable for 1 year at from date of receipt.

アナリシスノート

Control
Suggested Positive Antigen Control: Cat. # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20ºC. The lysate is stable for 6 months at -20ºC. Before use, add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.

法的情報

4G10 is a registered trademark of Upstate Group, Inc.

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable