07-1383

Anti-CDT1 Antibody

serum, from rabbit

• 別名: DNA replication factor , Double parked homolog , Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• クローン: polyclonal
• application: IP; WB
• 化学種の反応性: human, mouse, rat
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• citations: 4

特性

• 由来生物: rabbit
• 品質水準: 100
• 抗体製品の状態: serum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human, mouse, rat
• テクニック: immunoprecipitation (IP): suitable; western blot: suitable
• NCBIアクセッション番号: NP_112190.2
• UniProtアクセッション番号: Q9H211
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... CDT1(81620) ; mouse ... Cdt1(67177) ; rat ... Cdt1(292071)

詳細

詳細

CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006).

特異性

This antibody recognizes the Cdt1.

免疫原

Epitope: C-terminal half

Recombinant protein including amino acids 238-546 of human CDT1.

アプリケーション

Research Category
細胞シグナル伝達

Research Sub Category
細胞周期、DNA複製及び修復

An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000).

Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP.

品質

Evaluated by western blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.

ターゲットの説明

65 kDa

物理的形状

Unpurified rabbit serum containing 0.05% NaN3.

保管および安定性

Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1