おすすめの製品
由来生物
rabbit
品質水準
抗体製品の状態
affinity isolated antibody
抗体製品タイプ
primary antibodies
クローン
polyclonal
精製方法
affinity chromatography
化学種の反応性
human
メーカー／製品名
Upstate®
テクニック
western blot: suitable
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
phosphorylation (pTyr1148)
遺伝子情報
human ... EGFR(1956)
特異性
Predicted to cross-react with mouse and rat based on sequence homology (100%), but have not been tested.
Recognizes phospho-EGFR (Tyr1148)
免疫原
Peptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-EGFR (Tyr1148)
アプリケーション
Research Category
細胞シグナル伝達
細胞シグナル伝達
Research Sub Category
成長因子及び受容体
成長因子及び受容体
Immunoblot Analysis: A 1:1000 dilution of this lot detected phospho-EGFR (Tyr1148) in RIPA lysates from EGF stimulated A431 cells.
This Anti-phospho-EGFR (Tyr1148) Antibody is validated for use in WB for the detection of phospho-EGFR (Tyr1148).
品質
Routinely evaluated by immunoblot.
ターゲットの説明
185 kDa
物理的形状
ImmunoAffinity Purified
100 μl of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol.
保管および安定性
Stable for 2 years at -20°C from date of shipment.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
07-819:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Changes in retinal cell fate induced by overexpression of EGF receptor
Lillien, L.
Nature, 377, 158-162 (1995)
Polymorphonuclear leukocytes-mediated lysis of A431 cells induced by IgG1 mouse anti-epidermal growth factor receptor monoclonal antibodies.
Kawamoto, T, et al.
In Vitro Cellular & Developmental Biology, 28A, 782-786 (1992)
Wojciech Stec et al.
Oncotarget, 9(9), 8560-8572 (2018-03-02)
Despite intensive research no therapies targeted against the oncogenic EGFRvIII are present in the clinic. One of the reasons is the elusive nature of the molecular structure and activity of the truncated receptor. The recent publications indicate the EGF-bound wild-type
G R Johnson et al.
The Journal of biological chemistry, 268(4), 2924-2931 (1993-02-05)
The COOH-terminal half of the amphiregulin (AR) molecule has sequence homology to epidermal growth factor (EGF). The ability of AR to elicit in vivo phosphorylation of the EGF receptor (EGFR) and p185erbB2 was studied in four human epithelial cell lines
Cytotoxic properties of DAB486EGF and DAB389EGF, epidermal growth factor (EGF) receptor-targeted fusion toxins.
Shaw, J P, et al.
The Journal of Biological Chemistry, 266, 21118-21124 (1991)
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