14-326-M

Src Protein, active, 10 µg

Active, N-Terminal His6-tagged, recombinant, full-length, human Src. For use in Kinase Assays.

• 別名: SRC1
• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.26

特性

• 由来生物: human
• 品質水準: 100
• リコンビナント: expressed in Sf21 cells
• 形状: liquid
• 分子量: M_w 61.7 kDa
• メーカー／製品名: Upstate^®
• テクニック: activity assay: suitable (kinase)
• 溶解性: soluble
• NCBIアクセッション番号: NM_005417.3; NM_198291.1
• UniProtアクセッション番号: P12931
• 遺伝子情報: human ... SRC(6714)

詳細

詳細

Research area: Cell Signaling

N-Terminal His6-tagged, recombinant, full-length, human Src.

Src proteins are non-receptor protein tyrosine kinases, that belong to the Src family of kinases. These proteins have a unique structure consisting of an N-terminal region with a 14-carbon myristoyl group, two Src homology domains (SH2 and SH3), a catalytic tyrosine-protein kinase domain (SH1), and a short C-terminal tail. The SH1 domain is catalytic in function, while the SH2 and SH3 domains are non-catalytic and regulatory in nature.

Product Source: Human c-src, expressed in Sf21 cells.

アプリケーション

The Src protein may be used to study the interaction between CD133 and Src protein and its effect on FAK phosphorylation and cell migration through in vitro kinase assay.

生物化学的／生理学的作用

Src protein tyrosine kinases are involved in signaling pathways that control a plethora of cellular functions ranging from cell cycle, proliferation, differentiation, gene transcription, immune responses, apoptosis, etc. Upon its activation, Src phosphorylates and activates several other kinases Like MAPK (Mitogen-Activated Protein Kinase), p38, and ERK (Extracellular signal-regulated kinase). Activated MAPK (Mitogen-Activated Protein Kinase) regulates inflammation, cell development, differentiation and senescence. P38 is involved with the migration and survival of endothelial cells and extracellular signal-regulated kinase (ERK) promotes the proliferation and inflammation of endothelial cells. They play a key role in the regulation of cytoskeletal organization through the phosphorylation of substrates like Actin filament-associated protein 1 (AFAP1) and cortactin (CTTN). Src also plays a crucial role in maintaining cell adhesion, morphology, motility, and bone resorption by interacting with focal adhesion kinases. Src tyrosine kinase activity is upregulated in a variety of cancers including colon, lung, and pancreatic cancer, etc.

包装

2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-326M when ordering the 250μg size.

品質

Routinely evaluated by phosphorylation of Src Substrate Peptide

その他情報

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法的情報

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H317
• 注意書き: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危険有害性の分類: Skin Sens. 1
• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable