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Merck

SAB1306082

Sigma-Aldrich

ANTI-HIS TAG antibody produced in rabbit

purified immunoglobulin, buffered aqueous solution

別名:

Rabbit anti-hexa histidine tag

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.43

由来生物

rabbit

品質水準

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

polyclonal

フォーム

buffered aqueous solution

テクニック

western blot: 1:1000

UniProtアクセッション番号

輸送温度

wet ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

詳細

Histidine has a strong association with immobilized metal ion matrices. This is because the imidazole ring has electron donor groups that form coordination bonds with the transition metal that is immobilized. Hexahistidine tag (His-tag) consists of four to six histidines that are fused genetically. It is mainly used to purify proteins by immobilized metal-ion affinity chromatography (IMAC). His-tags are added at the amino or carboxy termini of proteins.

物理的形状

PBS(0.09% (W/V)アジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable


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[16] Purification of Proteins Using Polyhistidine Affinity Tags
Joshua A, et al.
Methods in Enzymology (2000)
Tandem Immobilized Metal-Ion Affinity Chromatography/Immunoaffinity Purification of His-tagged Proteins? Evaluation of Two Anti-His-Tag Monoclonal Antibodies
Kristian M.Muller, et al.
Analytical Biochemistry, 54-61 (1998)
Shuting Ding et al.
The EMBO journal, 42(6), e111858-e111858 (2022-12-24)
Phytosulfokine (PSK) is a plant pentapeptide hormone that fulfills a wide range of functions. Although PSK has frequently been reported to function in the inverse regulation of growth and defense in response to (hemi)biotrophic pathogens, the mechanisms involved remain largely
Yuria Jang et al.
International journal of molecular medicine, 53(1) (2023-12-08)
The binding between receptor‑activated nuclear factor‑κB (RANK) and the RANK ligand (RANKL) during osteoclast development is an important target for drugs that treat osteoporosis. The leucine‑rich repeat‑containing G‑protein‑coupled receptor 4 (LGR4) acts as a negative regulator of RANK‑RANKL that suppresses
Joshua Linnert et al.
Frontiers in cell and developmental biology, 11, 1199069-1199069 (2023-07-10)
The human Usher syndrome (USH) is the most common form of a sensory hereditary ciliopathy characterized by progressive vision and hearing loss. Mutations in the genes ADGRV1 and CIB2 have been associated with two distinct sub-types of USH, namely, USH2C

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