17-683
ChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set
clone CMA309, from mouse
別名:
H3K27Ac, Histone H3 (acetyl K27), Histone H3K27Ac
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About This Item
UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.32
クローン:
CMA309, monoclonal
application:
ChIP
IP
WB
IP
WB
化学種の反応性:
vertebrates, human
テクニック:
ChIP: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
26
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詳細
All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
The ChIPAb+ Acetyl-Histone H3 (Lys27) set includes the Anti-acetyl-Histone H3 (Lys27) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 178 bp region within the promoter of the human RPL10 gene. The acetyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of acetyl-histone H3 (Lys27)-associated chromatin.
The ChIPAb+ Acetyl-Histone H3 (Lys27) set includes the Anti-acetyl-Histone H3 (Lys27) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 178 bp region within the promoter of the human RPL10 gene. The acetyl-histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of acetyl-histone H3 (Lys27)-associated chromatin.
Lysine acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone acetylation is often associated with transcriptional activation and acetylation of H3K27 is found at active enhancers.
The previously assigned protein identifier Q66I33 has been merged into P84243. Full details can be found on the UniProt database.
特異性
Recognizes histone H3, Mr 17 kDa, acetylated at Lys27.
This peptide sequence is identical in a wide range of animal and plant species.
免疫原
Epitope: a.a. 19-37
The acetyl-histone H3 (Lys27) purified antibody is made against a synthetic peptide (acetylated at Lys27) corresponding to amino acids 19-37 of histone H3.
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
エピジェネティクス
エピジェネティクス
Acetyl-Histone H3 (Lys27) ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of H3K27Ac.
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-acetyl-Histone H3 (Lys27) antibody and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys27)-associated DNA fragments was verified by qPCR using β-globin Promoter ChIP Primers versus RPL10 Promoter Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Acid extracts from untreated (Lane 1) and sodium-butyrate treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-acetyl Histone H3 (Lys27), clone CMA309 (0.1 μg/mL). Proteins were visualized using a goat
anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-acetyl-Histone H3 (Lys27) antibody and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of acetyl-histone H3 (Lys27)-associated DNA fragments was verified by qPCR using β-globin Promoter ChIP Primers versus RPL10 Promoter Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna G ChIP (Cat. #17-408) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Western Blot Analysis:
Acid extracts from untreated (Lane 1) and sodium-butyrate treated (Lane 2) HeLa cells were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-acetyl Histone H3 (Lys27), clone CMA309 (0.1 μg/mL). Proteins were visualized using a goat
anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
包装
25 assays per kit, ~2μg per chromatin immunoprecipitation
品質
Chromatin Immunoprecipitation:
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-acetyl-Histone H3 (Lys27) antibody and the Magna ChIP G (Cat. #17-611) Kit.
Successful immunoprecipitation of acetyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers RPL10 Promoter (Please see figures).
Please refer to the EZ-Magna G ChIP (Cat. #17-409) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-acetyl-Histone H3 (Lys27) antibody and the Magna ChIP G (Cat. #17-611) Kit.
Successful immunoprecipitation of acetyl-histone H3 (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers RPL10 Promoter (Please see figures).
Please refer to the EZ-Magna G ChIP (Cat. #17-409) or EZ-ChIP (Cat. #17-371) protocol for experimental details.
ターゲットの説明
~17 kDa
物理的形状
Anti-acetyl-Histone H3 (Lys27) (mouse monoclonal IgG1, Clone CMA309). One vial containing 50 μg of protein G purified antibody in 50 μL PBS containing 0.05% sodium. Store at -20°C.
Normal Mouse IgG. Two vials containing 25 μg purified Mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, RPL10 Promoter. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human RPL10. Store at -20°C.
FOR: ACC CGT CTT CGA CAG GAC T
REV: GGA ACG GAA GAC GAG AAC AG
Normal Mouse IgG. Two vials containing 25 μg purified Mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.
ChIP Primers, RPL10 Promoter. One vial containing 75 μL of 5 μM of each primer specific for the promoter region of human RPL10. Store at -20°C.
FOR: ACC CGT CTT CGA CAG GAC T
REV: GGA ACG GAA GAC GAG AAC AG
Format: Purified
保管および安定性
Stable for 1 year at -20°C from date of receipt. Aliquot upon thawing, avoid freeze thaw cycles.
アナリシスノート
Control
Included negative control mouse IgG antibody and control primers specific for human RPL10 promoter.
Included negative control mouse IgG antibody and control primers specific for human RPL10 promoter.
法的情報
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
保管分類コード
10 - Combustible liquids
適用法令
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試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Marta Kubiak et al.
International journal of molecular sciences, 20(2) (2019-01-13)
The long-range control of gene expression is facilitated by chromatin looping and can be detected using chromosome conformation capture-3C. Here we focus on the chromatin architecture of the PTBP3 (Polypyrimidine tract binding protein 3) locus to evaluate its potential role
Chao Yang et al.
Molecular plant, 13(3), 515-531 (2020-02-23)
Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development, but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants. In this study
Katherine A Pillman et al.
Nucleic acids research, 47(16), 8606-8619 (2019-08-03)
Epithelial-mesenchymal transition (EMT) has been a subject of intense scrutiny as it facilitates metastasis and alters drug sensitivity. Although EMT-regulatory roles for numerous miRNAs and transcription factors are known, their functions can be difficult to disentangle, in part due to
Akua Yalley et al.
The Journal of biological chemistry, 291(16), 8848-8861 (2016-03-02)
FoxO1 binds to insulin response elements located in the promoters of insulin-like growth factor-binding protein 1 (IGFBP1) and glucose-6-phosphatase (G6Pase), activating their expression. Insulin-mediated phosphorylation of FoxO1 promotes cytoplasmic translocation, inhibiting FoxO1-mediated transactivation. We have previously demonstrated that FoxO1 opens
Gabrielle Gay et al.
The Journal of experimental medicine, 213(9), 1779-1798 (2016-08-10)
An early hallmark of Toxoplasma gondii infection is the rapid control of the parasite population by a potent multifaceted innate immune response that engages resident and homing immune cells along with pro- and counter-inflammatory cytokines. In this context, IFN-γ activates
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