208748

Calpain-1 Substrate, Fluorogenic

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

• 別名: Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
• 実験式（ヒル表記法）: C₇₉H₉₅N₁₃O₁₉S₂
• 分子量: 1594.81
• UNSPSCコード: 12352200
• NACRES: NA.77

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• 形状: lyophilized solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated (hygroscopic); protect from light
• 色: red
• 溶解性: DMSO: 1 mg/mL
• 蛍光検出: λ_ex ~490 nm; λ_em ~518 nm
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs between Tyr-Gly residues and results in enhanced fluorescence.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

シーケンス

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

物理的形状

Supplied as a trifluoroacetate salt.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable