324696

Elastase Substrate I, Colorimetric

A specific chromogenic substrate for human neutrophil elastase.

• 別名: Elastase Substrate I, Colorimetric
• 実験式（ヒル表記法）: C₂₇H₃₈N₆O₉
• CAS番号: 70967-90-7
• 分子量: 590.63
• MDL番号: MFCD00077137
• UNSPSCコード: 12352200
• NACRES: NA.54

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: lyophilized
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; desiccated
• 溶解性: water: 1 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C
• SMILES記法: [N+](=O)([O-])c1ccc(cc1)N[C@@H](C(C)C)C(=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)OC)C)C
• InChI: 1S/C27H38N6O9/c1-15(2)23(30-18-8-10-19(11-9-18)33(40)41)26(38)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23,30H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,31,37,38)/t16-,17-,20-,23-/m0/s1
• InChI Key: JWYKDZIEIWGSBN-OSAZLGQLSA-N

詳細

詳細

A specific chromogenic substrate for human neutrophil elastase (K_cat/K_m = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.

Specific chromogenic substrate for human neutrophil elastase (k_cat/K_m = 120,000 versus pancreatic elastase: k_cat/K_m = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.

生物化学的／生理学的作用

Cell permeable: no

Kcat/Km = 120,000 vs. 2700 for pancreatic elastase

Primary Target
Chromogenic substrate for human neutrophil elastase

Product does not compete with ATP.

Reversible: no

警告

Toxicity: Standard Handling (A)

シーケンス

MeOSuc-Ala-Ala-Pro-Val-pNA

物理的形状

Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

その他情報

Barrett, A.J., et al. 1989. Biochim. J.260, 259.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable