480708

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

• 別名: α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase
• CAS番号: 9001-67-6
• Enzyme Commission number: 3.2.1.18 (BRENDA, IUBMB)
• UNSPSCコード: 12352202
• NACRES: NA.54

特性

• リコンビナント: expressed in E. coli
• 品質水準: 100
• 製品種目: EMPROVE^® EXPERT
• 形状: liquid
• 比活性: ≥10 units/mL; ≥250 units/mg protein
• メーカー／製品名: Calbiochem^®
• 保管条件: do not freeze
• その他の活性: N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

Note: 1 mU = 1 milliunit.

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

アプリケーション

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

警告

Toxicity: Harmful (C)

単位の定義

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

物理的形状

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

その他情報

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• ピクトグラム: GHS05,GHS07
• シグナルワード: Warning
• 危険有害性情報: H290 - H315 - H319 - H335
• 注意書き: P234 - P261 - P264 - P271 - P302 + P352 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 8B - Non-combustible, corrosive hazardous materials
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable