70922

BugBuster^® HT Protein Extraction Reagent

• 別名: Cell lysis reagent, Protein extraction buffer
• UNSPSCコード: 41106511
• NACRES: NA.77

特性

• 形状: liquid
• 品質水準: 100
• メーカー／製品名: Novagen^®
• 保管条件: OK to freeze; avoid repeated freeze/thaw cycles
• 輸送温度: wet ice
• 保管温度: 2-8°C

詳細

詳細

BugBuster HT Protein Extraction Reagent combines BugBuster Protein Extraction Reagent and Benzonase^® Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.

アプリケーション

BugBuster^® HT Protein Extraction Reagent has been used:

• to lyse the Escherichia coli cell pellets following centrifugation for protein expression and immunoblotting
• for protein extraction from bacterial cell pellet
• used to lyse the E. coli cell pellets for the expression and purification of dMIC60 (mitofilin) protein

特徴および利点

• Fast protein extraction and nucleic acid degradation
• Ideal for processing various samples of different volumes
• Increased extraction is ensured by the addition of rLysozyme solution

警告

Toxicity: Standard Handling (A)

法的情報

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable